Antibacterial and Biofilm Degradation Activity of Extract From Steam Distillation Residue of Zingiberaceae Leaves Against Streptococcus mutans

Keywords: antibacterial, biofilm degradation, Streptococcus mutans, Zingiberaceae leaves, Distillation residue

Abstract

Zingiberaceae is a family of plant that has been widely used to treat various diseases and as an element of spice in cooking. In this paper, the potential of the extract from the steam distillation residue of Zingiberaceae leaves as antibacterial and biofilm degradation agent was studied and determined against Streptococcus mutans. Five different species of Zingiberaceae, which consisted of Curcuma longa, Curcuma zeodoaria, Curcuma aeruginosa, Zingiber officinale, Zingiber cassumunar were taken for samples and their distillation residues were extracted by soxhlation using 3 different solvents namely n-hexane, ethyl acetate, and methanol. The antibacterial and biofilm degradation activity of the assay from each of the samples was determined by the microdilution technique. Among the 15 Zingiberaceae leaves distillation residue extracts, five are categorically active against Streptococcus mutans with their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values being the same with that of chloramphenicol, 15.63 μg/mL. All extracts were found to degrade the biofilm. The methanol extract of C. zeodoaria leaves was found to have the highest antibacterial activity with MIC and MIB vaues of 15.63 ppm and the best to degrade the biofilm with inhibitory concentration 50% (IC50) of 15.33 ppm. The antibacterial and biofilm degradation activities of extracts are not related to the phenolic content and it was suggested that terpenoid such as (-)-zingiberene may have been the active component.

Downloads

Download data is not yet available.

References

I. Batubara, W.T. Wahyuni and M. Susanto, Antibacterial Activity of Zingiberaceae Leaves Essential Oil Against Streptococcus mutans and Teeth-biofilm Degradation, IJPBS, 2016, 7, 111-116. DOI: https://doi.org/10.22376/ijpbs.2016.7.4.p111-116.

I. Batubara, U. Zahra, L.K. Darusman and A. Maddu, Minyak Atsiri Daun Zingiberaceae sebagai Antioksidan dan Antiglikasi, IJEO, 2016, 1, 44-52.

S. Sindhu, B. Chempakam, N.K. Lellla and R.S. Bhai, Chemoprevention by Essential Oil of Turmeric Leaves (Curcuma longa L.) on The Growth of Aspergillus flavus and Aflatoxin Production, FCT, 2011, 49, 1188-1192. DOI: https://doi.org/10.1016/j.fct.2011.02.014.

H.O. Edeoga. Phytochemical constituents of some Nigerian medicinal Plants. Afr J Biotechnol. 2005;4:685–8. DOI: https://doi.org/10.5897/AJB2005.000-3127

G.A. Ayoola. Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in southwestern Nigeria. Trop J Pharm Res. 2008;7:1019–24. DOI: https://doi.org/10.4314/tjpr.v7i3.14686

U. Zahra, Y. Kartika, I. Batubara, L.K. Darusman and A. Maddu, Short Communication: Screening The Potency of Zingiberaceae Leaves as Antioxidant and Antiaging Agent. Nus. Biosci., 2016, 8, 221-225, DOI: https://doi.org/10.13057/nusbiosci/n080214.

S. Mongkolsilp, I. Pongbupakit, N. Sae-lee and W. Sithithaworn, Radical Scavenging Activity and Total Phenolic Content of Medicinal Plants Used in Primary Health Care, J. Pharm. Sci., 2004, 9, 32-35.

S. Kaur and P. Mondal, Study of Total Phenolic and Flavonoid Content, Antioxidant Activity and Antimicrobial Properties of Medicinal Plants. J. Microbiol Exp., 2014, 1, 23-28, DOI: https://doi.org/10.15406/jmen.2014.01.00005.

R. Lobo, K.S. Prabhu, A. Shirwaikar and A. Shirwaikar, Curcuma zedoaria Rosc. (White Turmeric): A Review of Its Chemical, Pharmacological and Ethnomedicinal Properties, J. Pharm. Pharmacol., 2009, 61, 13-21, DOI: https://doi.org/10.1211/jpp/61.01.0003.

R. Ahmad, S.N. Baharum, H. Bunawan, M. Lee, N.M. Noor, E.R. Rohani, N. Ilias and N.M. Zin, Volatile Profiling of Aromatic Traditional Medicinal Plant, Polygonum minus in Different Tissues and Its Biological Activities. Molecules, 2014, 19, 19220-19242, DOI: https://doi.org/10.3390/molecules191119220.

Extract from the steam distillation residue of Zingiberaceae leaves was used as antibacterial and biofilm degradation agent against Streptococcus mutans using microdilution technique.  The methanol extract of C. zeodoaria leaves shows the highest antibacterial activity with MIC and MIB vaues of 15.63 ppm and the best to degrade the biofilm with inhibitory concentration 50% (IC50) of 15.33 ppm.
Published
2019-08-31
How to Cite
Batubara, I., Yunita, D., & Suparto, I. H. (2019). Antibacterial and Biofilm Degradation Activity of Extract From Steam Distillation Residue of Zingiberaceae Leaves Against Streptococcus mutans . Journal of the Indonesian Chemical Society, 2(1), 42. https://doi.org/10.34311/jics.2019.02.1.42