Secondary Metabolites from Indonesian Kigelia africana (Bignoniaceae)

  • Purwantiningsih Sugita Department of Chemistry, IPB University, Indonesia http://orcid.org/0000-0002-0305-8123
  • Dina Anggraini Department of Chemistry, IPB University, Indonesia
  • Gustini Syahbirin Department of Chemistry, IPB University, Indonesia
  • Dyah Utami Cahyaning Rahayu Department of Chemistry, Universitas Indonesia, Indonesia
  • Auliya Ilmiawati Department of Chemistry, IPB University, Indonesia
DOI: https://doi.org/10.34311/jics.2019.02.2.114
Keywords: Kigelia africana, Anticancer activity, Methyl ferulate (CD-1), CD-2 and B-1 fractions

Abstract

From the fruit of Kigelia africana three fractions of secondary metabolites were isolated. Fraction 1 (CD-1) was characterized by using UV-Vis, FTIR, 1D and 2D NMR and MS, meanwhile fraction 2 (CD-2) and 3 (B-1) were characterized by LCMS and compared with literature. All these fractions were screened for anticancer activity by using (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method to calculate IC50 toward Michigan Cancer Foundation-7 MCF-7 breast cancer and menogaril-resistant mouse leukemia P388 cells. Based on spectroscopic data, CD-1 fraction was identified as methyl ferulate (1). On the other hand, the B-1 and CD-2 fraction did not pure, yet. Based on LCMS data that analyzed by chemspider and masslink software, CD-2 fraction was estimated as compound mixture with dominant compounds like viscumside (2), specioside (3), caffeic acid glucoside (4), ferulic acid (5), meanwhile B-1 fraction was estimated as compound mixture with dominant warfarin alcohol (6), p-Coumaroyl glucose (7) and β - Sitosterol (8) compounds. Nevertheless, all isolated fractions did not show anticancer activity towards both the cancer cells since it had different constituent compared with previous results.

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CD-1, CD-2 and B-1 fractions isolated from Indonesian fruit of Kigelia africana were test for its anticancer activity. The plant was collected from Botanical Garden, Bogor Indonesia. All fractions did not show anticancer activity for both MCF-7 breast cancer and P-388 murin leukemia cells. In contrast, the previous research, fractions isolated from EtOAc extract of K. africana which was collected from PT. Alam Indah Bunga Nusantara, Cianjur, Indonesia showed relatively high inhibitions (above 80%) against cancer cells MCF-7. The difference in results due to compounds identified by LCMS were different. The identified compounds were 5,7-dimethoxy-4-methyl coumarin, 3-hydroxy-β-lapachone, 7H, 8H-dipenalen-9,2-b:2,1-difuran-7.8- dione, and 3’,6-dimethyl flavone. Therefore the anticancer activity was also different.
Published
2019-12-30
How to Cite
Sugita, P., Anggraini, D., Syahbirin, G., Rahayu, D. U. C., & Ilmiawati, A. (2019). Secondary Metabolites from Indonesian Kigelia africana (Bignoniaceae). Journal of the Indonesian Chemical Society, 2(2), 114. https://doi.org/10.34311/jics.2019.02.2.114
Issue
Vol 2 No 2 (2019): JICS, 30 December 2019 (pp 66-125)
Section
Full Paper

Copyright (c) 2019 Purwantiningsih Sugita, Dina Anggraini, Gustini Syahbirin, Dyah Utami Cahayaning Rahayu, Auliya Ilmiawati

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