Distribution of Chlorophylls and Carotenoids in the Different Parts of Thallus Structure from Three Sargassum spp. as Revealed by Multi-Chromatograms HPLC Approach

  • Renny Indrawati Ma Chung Research Center for Photosynthetic Pigments, Universitas Ma Chung, Malang, Indonesia
  • Heriyanto Ma Chung Research Center for Photosynthetic Pigments, Universitas Ma Chung, Malang, Indonesia
  • Tatas H. P. Brotosudarmo Ma Chung Research Center for Photosynthetic Pigments, Universitas Ma Chung, Malang, Indonesia
  • Leenawaty Limantara Center for Urban Study, Universitas Pembangunan Jaya, Tangerang Selatan, Banten, Indonesia
Keywords: Sargassum sp., chlorophylls, carotenoids, HPLC-PDA, multi-chromatograms


Macroalgae such as Sargassum sp. has a unique morphology. Its thallus consists of three different parts: stem-like, leaf-like, and vesicles. Each part of the thallus contains photosystems, which support its photosynthetic growth. Here we report the distribution of chlorophylls and carotenoids in the different part of thallus from three Sargassum spp. variants.  We found that the dominant pigments are chlorophyll a and fucoxanthin. The chlorophyll c, siphonein, violaxanthin, flavoxanthin, zeaxanthin, and β-carotene were also found as an accessory pigment. Prior to analysis, we develop a multi-chromatograms approach by employing reversed-phase high-performance liquid chromatography (HPLC) with photodiode array (PDA) detection, which able to extract directly spectroscopic data from 350 to 700 nm wavelengths.


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Author Biography

Leenawaty Limantara, Center for Urban Study, Universitas Pembangunan Jaya, Tangerang Selatan, Banten, Indonesia

Ma Chung Research Center for Photosynthetic Pigments, Universitas Ma Chung, Malang, Indonesia


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