Distribution of Chlorophylls and Carotenoids in the Different Parts of Thallus Structure from Three Sargassum spp. as Revealed by Multi-Chromatograms HPLC Approach

  • Renny Indrawati Ma Chung Research Center for Photosynthetic Pigments, Universitas Ma Chung, Malang, Indonesia
  • Heriyanto Ma Chung Research Center for Photosynthetic Pigments, Universitas Ma Chung, Malang, Indonesia
  • Tatas H. P. Brotosudarmo Ma Chung Research Center for Photosynthetic Pigments, Universitas Ma Chung, Malang, Indonesia
  • Leenawaty Limantara Center for Urban Study, Universitas Pembangunan Jaya, Tangerang Selatan, Banten, Indonesia
Keywords: Sargassum sp., chlorophylls, carotenoids, HPLC-PDA, multi-chromatograms

Abstract

Macroalgae such as Sargassum sp. has a unique morphology. Its thallus consists of three different parts: stem-like, leaf-like, and vesicles. Each part of the thallus contains photosystems, which support its photosynthetic growth. Here we report the distribution of chlorophylls and carotenoids in the different part of thallus from three Sargassum spp. variants.  We found that the dominant pigments are chlorophyll a and fucoxanthin. The chlorophyll c, siphonein, violaxanthin, flavoxanthin, zeaxanthin, and β-carotene were also found as an accessory pigment. Prior to analysis, we develop a multi-chromatograms approach by employing reversed-phase high-performance liquid chromatography (HPLC) with photodiode array (PDA) detection, which able to extract directly spectroscopic data from 350 to 700 nm wavelengths.

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Author Biography

Leenawaty Limantara, Center for Urban Study, Universitas Pembangunan Jaya, Tangerang Selatan, Banten, Indonesia

Ma Chung Research Center for Photosynthetic Pigments, Universitas Ma Chung, Malang, Indonesia

References

[1] Burtin, P. (2003), Nutritional Value of Seaweeds. Electronic Journal of Environmental, Agricultural and Food Chemistry. vol 2 (4), 498 – 503.
[2] Radmer, R. J. (1996), Algal Diversity and Commercial Algal Products. BioScience Marine Biotechnology. vol 46 (4), 263 – 270.
[3] Wang, X-F., C-H Zhan, T. Maoka, Y. Wada, and Y. Koyama. (2007), Fabrication of Dye-Sensitized Solar Cells using Chlorophylls c1 and c2 and Their Oxidized Forms and from Undaria pinnatifida (Wakame). Chemical Physics Letters. vol 447 (1-3), 79 – 85.
[4] Wang, X-F., Y. Koyama, O. Kitao, Y. Wada, S. Sasaki, H. Tamiaki, and H. Zhou. (2010), Significant Enhancement in the Power-Conversion Efficiency of Chlorophyll Co-Sensitized Solar Cells by Mimicking the Principles of Natural Photosynthetic Light-Harvesting Complexes. Biosensors and Bioelectronics. vol 25 (8), 1970 – 1976.
[5] Kuo, C-G. and B-J. Sheen. (2011). Seaweed Chlorophyll on the Light-electron Efficiency of DSSC. Journal of the Chinese Chemical Society. vol 58 (2). 186 – 190.
[6] Yan, X., Y. Chuda, M. Suzuki, and T. Nagata. (1999) Fucoxanthin as the Major Antioxidant in Hijikia fusiformis, Common Edible Seaweed. Bioscience, Biotechnology, and Biochemistry. vol 63 (3), 605 – 607.
[7] Maeda, H., M. Hosokawa, T. Sashima, K. Funayama, and K. Miyashita. (2005). Fucoxanthin from Edible Seaweed, Undaria pinnatifida, Shows Antiobesity Effect through UCP1 Expression in White Adipose Tissues. Biochemical and Biophysical Research Communications. vol 332 (2), 392 – 397.
[8] Maeda, H., T. Tsukui, T. Sashima, M. Hosokawa, and K. Miyashita. (2008). Seaweed Carotenoid, Fucoxanthin, as a Multi-functional Nutrient. Asia Pacific Journal of Clinical Nutrition. vol 17 (S1), 196 – 199.
[9] Kilar, J. A., M. M. Littler, and D. S. Littler. (1989). Functional – Morphological Relationship in Sargassum polyceratium (Phaeophyta): Phenotypic and Ontogenetic Variability in Apparent Photosynthesis and Dark Respiration. Journal of Phycology. vol 25, 713 – 720.
[10] Gao, K. and I. Umezaki. (1988). Comparative Photosynthetic Capacities of the Leaves of Upper and Lower Parts of Sargassum Plants. Botanica Marina. vol 31, 231 – 236.
[11] Gao, K. and I. Umezaki. (1989). Comparative Studies of Photosynthesis in Different Parts of Sargassum thunbergii. Japanese Journal of Phycology. vol 37, 7 – 16.
[12] Garbary, D. J. and K. Y. Kim. (2005). Anatomical Differentiation and Photosynthetic Adaptation in Brown Algae. Algae. vol 20 (3), 233 – 238.
[13] Terasaki, M., A. Hirose, B. Narayan, Y. Baba, C. Kawagoe, H. Yasui, N. Saga, M. Hosokawa, and K. Miyashita. (2009). Evaluation of Recoverable Functional Lipid Components of Several Brown Seaweeds (Phaeophyta) from Japan with Special Reference to Fucoxanthin and Fucosterol Contents. Journal of Phycology. vol 45 (4), 974 – 980.
[14] Nielsen, N-P. V., J. M. Carstensen, and J. Smedsgaard. (1998). Aligning of Single and Multiple Wavelength Chromatographic Profiles for Chemometric Data Analysis using Correlation Optimised Warping. Journal of Chromatography A. vol 805, 17 – 35.
[15] Indrawati, R., Heriyanto, L. Limantara, and A. B. Susanto. (2010). Study of Pigments Distribution in the Stem, Leaf and Vesicle of Sargassum filipendula C. Agardh, Sargassum polycystum C. Agardh and Other Sargassum spp. from Madura Waters by High-Performance Liquid Chromatography. Proceedings of Natural Pigments Conference for South-East Asia (NP-SEA). Ma Chung University. Indonesia. 225 – 230.
[16] Hegazi, M. M., A. Pérez-Ruzafa, L. Almela, M. E. Candela. (1998). Separation and Identification of Chlorophylls and Carotenoids from Caulerpa prolifera, Jania rubens and Padina pavonica by Reversed-Phase High-Performance Liquid Chromatography. Journal of Chromatography A. vol 829, 153 – 159.
[17] Scheer, H. (2006), Chapter 1. An Overview of Chlorophylls and Bacteriochlorophylls: Biochemistry, Biophysics, Functions and Applications, Chlorophylls and Bacteriochlorophylls. vol 25, 1-26.
[18] Gross, J. (1991), Pigments in Vegetables: Chlorophylls and Carotenoids. Van Nostrand Reinhold. USA.
[19] Sambarimurty, A. V. S. S. (2005), A Textbook of Algae. I. K. International. India.
[20] Choudhury, N. K. and R. K. Behera. (2001), Photoinhibition of Photosynthesis: Role of Carotenoids in Photoprotection of Chloroplast Constituents. Photosynthetica. vol 39 (4), 481 – 488.
[21] Wright, S. W. and S. W. Jeffrey. 2005. Pigment Markers for Phytoplankton Production, Marine Organic Matter: Biomarkers, Isotopes and DNA. Hdb Env Chem. vol 2, 71 – 104.
Published
2019-06-28