Preliminary Study of the Potential of Phanerochaete chrysosporium Immobilized in Agar for Degradation of Sugarcane Bagasse
Previous research has shown that the culture of Phanerochaete chrysosporium in stationary phase is able to degrade sugarcane bagasse to glucose in a single reaction step. The aims of this study is to study the ability of P. chrysosporium immobilized in agar with or without rejuvenation to degrade bagasse and to determine the optimum process conditions comprising method, amount of bagasse, temperature and incubation time. The immobilized P. chrysosporium without rejuvenation was incubated with bagasse directly, while the immobilized P. chrysosporium within rejuvenation first incubated in N-limited growth media for 48 hours and washed before being incubated with bagasse. Glucose as a parameter to determine the ability of degradation is determined by the Somogy-Nelson method. The yield of bagasse degradation by the immobilized P. chrysosporium without or with the rejuvenation was 1.78% and 1.07% respectively, with 2g bagasse, for 7 days incubated, at 37°C, and shaking rate of 50 rpm. The optimum condition of bagasse degradation was conducted by the immobilized stationary phase P. chrysosporium (0.065 g dry weight) in 3.3% agar without rejuvenation for as much as 4 g of bagasse, the incubation temperature of 37 ° C, for 7 days, giving degradation rate of 1.045%.
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